Evaluation of the enzymatic inhibition of Tyrosinase
| Regulatory reference: | Proprietary method |
| Claim: | Depigmenting action |
| Application field: | Active ingredients, mixtures or finished products. |
| Description of the test: | Evaluation of the efficacy of a sample in inhibiting the activity of the tyrosinase enzyme in a controlled in vitro direct reaction. |
Evaluation of the modulation of melanogenesis on pigmented cells
| Regulatory reference: | Proprietary method |
| Claim: | Skin whitening and Depigmenting action |
| Application field: | Active ingredients or finished products. |
| Description of the test: | Quantification of the inhibition of melanin synthesis in human melanoma cells exposed to the substance or product to be tested, in comparison with untreated controls. The same test can also be used to quantify an increase of melanin synthesis. |
Evaluation of depigmenting efficacy
| Regulatory reference: | Guidelines to Commission Regulation (EU) No 655/2013 |
| Claim: | Reduces skin blemishes, Skin whitening and Depigmenting action |
| Application field: | Visage and body cosmetic products. |
| Description of the test: | Instrumental measurements performed using a Minolta CR200 colorimeter will be performed at T=0 and T=X days (to be defined by previous agreement with the sponsor) in order to evaluate changes in the pigmentation of the treated area. The measurements will be performed at each time point in the same area. Acquisition of high definition images will be performed at T=0 and T=X days using a macro objective with color normalization and coaxial circular flash. |
Evaluation of the efficacy of a product in increasing pigmentation and stimulating melatonin production with and without sunlight exposure
| Regulatory reference: | Guidelines to Commission Regulation (EU) No 655/2013 |
| Claim: | Tanner |
| Application field: | Visage and body cosmetic products. |
| Description of the test: | A first set of baseline measurements will be performed using a Minolta CR200 Colorimeter in order to identify the phototype of the target skin region and its correlated MEDu. The measurement will be repeated at T=0 and T=X days in order to evaluate changes in skin pigmentation. All measurements will be taken in the same target region. After the treatment, treated and untreated areas will be exposed to 3 irradiation cycles across 5 days using a tanning lamp, in order to quantify the increase in pigmentation. Measurements will be performed using a Colorimeter. High resolution imaging will be performed using a macro objective with coaxial circular flash, after each cycle of simulated sunlight exposure. |
Evaluation of the efficacy in the modulation of melanogenesis in pigmented cells by quantification of the expression of the tyrosinase gene
| Regulatory reference: | Proprietary method |
| Claim: | Skin whitening and Depigmenting action |
| Application field: | Active ingredients or finished products. |
| Description of the test: | Evaluation of the changes in the expression of the tyrosinase gene in human melanoma cells. |
Evaluation of the efficacy in the modulation of melanogenesis in pigmented cells
| Regulatory reference: | Proprietary method |
| Claim: | Tanner |
| Application field: | Active ingredients or finished products. |
| Description of the test: | Quantification of the increase of melanin synthesis in human melanoma cells treated with the sample substance or product in comparison with untreated controls. The same test can be used to quantify a reduction in melanin synthesis. |
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