Evaluation of the efficacy of a product in increasing pigmentation and stimulating melatonin production with and without sunlight exposure
| Regulatory reference: | Guidelines to Commission Regulation (EU) No 655/2013 |
| Claim: | Tanner |
| Application field: | Visage and body cosmetic products. |
| Description of the test: | A first set of baseline measurements will be performed using a Minolta CR200 Colorimeter in order to identify the phototype of the target skin region and its correlated MEDu. The measurement will be repeated at T=0 and T=X days in order to evaluate changes in skin pigmentation. All measurements will be taken in the same target region. After the treatment, treated and untreated areas will be exposed to 3 irradiation cycles across 5 days using a tanning lamp, in order to quantify the increase in pigmentation. Measurements will be performed using a Colorimeter. High resolution imaging will be performed using a macro objective with coaxial circular flash, after each cycle of simulated sunlight exposure. |
Anti-cellulite efficacy test through analysis of morphological changes
| Regulatory reference: | Guidelines to Commission Regulation (EU) No 655/2013 |
| Claim: | Reduces the esthetic effects of cellulite and Toning and firming action |
| Application field: | Body cosmetic products. |
| Description of the test: | Morphological analysis of the areas treated with the product by blue laser DermaTOP-V3 body scansion. The instrument can precisely quantify the changes in the volume of panniculus adiposus volumes such as micro and macro-nodules. |
Evaluation of skin smothness, elasticity and compactness
| Regulatory reference: | Guidelines to Commission Regulation (EU) No 655/2013 |
| Claim: | Anti-aging, Reduces signs of aging and Illuminating |
| Application field: | Visage products for mature skin. |
| Description of the test: | Instrumental evaluation of R0, R2 and R9 parameters. Instrument: MPA580 Cutometer® for the determination of skin viscoelasticity. Manufacturer: Courage-Khazaka GmbH (Germany). The L* parameter is also quantified for skin brightness. The effect is quantified through reflection colorimetry using a CL-200 Chromameter Measurements of parameters L* and b* are performed before and after the application of the product. |
Posted in Anti age, Anti-aging, Illuminating, Reduces signs of aging, Claim for cosmetics
Evaluation of skin compactness and elasticity
| Regulatory reference: | Guidelines to Commission Regulation (EU) No 655/2013 |
| Claim: | Firming efficacy, Improves skin tone and smoothness and Improves skin elasticity |
| Application field: | Visage and body products. |
| Description of the test: | Measurement of R0 and R2 parameters on 20 healthy volunteers. Instrument: MPA580 Cutometer® for the scientific quantification of the viscoelasticity of the skin. Manufacturer: Courage-Khazaka GmbH (Germany). |
Evaluation of the activation of the synthesis of collagen and fibronectin
| Regulatory reference: | Proprietary method |
| Claim: | Activates the synthesis of collagen, elastin, fibronectin |
| Application field: | Finished products and active ingredients. |
| Description of the test: | Real time PCR analysis of the expression of collagen and fibronectin, compared to an untreated control, to determine the efficacy of a product or ingredient in activating the synthesis of these proteins involved in maintaining skin elasticity and prevent aging. |
Direct antioxidant efficacy assay
| Regulatory reference: | Proprietary method |
| Claim: | Antioxidant |
| Application field: | Finished products and substances. |
| Description of the test: | Colorimetric evaluation of the antioxidant activity of the sample in solution on a controlled oxidation reaction. Results in terms of percentage of decrease of oxidation processes caused by free oxygen radicals. |
Posted in Anti age, Antioxidant, Claim for cosmetics
OECD 442D in vitro sensitization test
| Regulatory reference: | OECD 442D |
| Claim: | No skin sensitizing and Hypoallergenic |
| Application field: | Substances or mixtures. |
| Description of the test: | The ARE-Nrf2 method employs cytofluorometry to quantify the antioxidant response in a culture of human keratinocytes after exposure to the product. |
OECD 442E in vitro sensitization test
| Regulatory reference: | OECD 442E |
| Claim: | No skin sensitizing and Hypoallergenic |
| Application field: | Substances or mixtures. |
| Description of the test: | H-CLAT cytometry method to evaluate possible changes in the expression of cell surface markers (CD86 e CD54) in cultures of THP-1 human monocytes to exclude the related sensitization pathway. |
Ames test
| Regulatory reference: | OECD 471 |
| Claim: | Non mutagenic |
| Application field: | Raw ingredients for medical devices or cosmetics. |
| Description of the test: | Analysis of genotoxicity of a substance according to OECD 471. |
DPRA: Direct Peptide Reactivity Assay test
| Regulatory reference: | OECD 442C |
| Claim: | No skin sensitizing and Hypoallergenic |
| Application field: | Substances or mixtures without metal compounds. |
| Description of the test: | The DPRA is proposed to address the molecular initiating event of the skin sensitisation AOP (Adverse Outcome Pathway), namely protein reactivity, by quantifying the reactivity of test chemicals towards model synthetic peptides containing either lysine or cysteine. Cysteine and Lysine percent peptide depletion values are then used to categorise a substance in one of four classes of reactivity for supporting the discrimination between skin sensitisers and non-sensitisers. |
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