Evaluation of the efficacy of a product in increasing pigmentation and stimulating melatonin production with and without sunlight exposure
| Regulatory reference: | Guidelines to Commission Regulation (EU) No 655/2013 |
| Claim: | Tanner |
| Application field: | Visage and body cosmetic products. |
| Description of the test: | A first set of baseline measurements will be performed using a Minolta CR200 Colorimeter in order to identify the phototype of the target skin region and its correlated MEDu. The measurement will be repeated at T=0 and T=X days in order to evaluate changes in skin pigmentation. All measurements will be taken in the same target region. After the treatment, treated and untreated areas will be exposed to 3 irradiation cycles across 5 days using a tanning lamp, in order to quantify the increase in pigmentation. Measurements will be performed using a Colorimeter. High resolution imaging will be performed using a macro objective with coaxial circular flash, after each cycle of simulated sunlight exposure. |
Evaluation of the efficacy in the modulation of melanogenesis in pigmented cells
| Regulatory reference: | Proprietary method |
| Claim: | Tanner |
| Application field: | Active ingredients or finished products. |
| Description of the test: | Quantification of the increase of melanin synthesis in human melanoma cells treated with the sample substance or product in comparison with untreated controls. The same test can be used to quantify a reduction in melanin synthesis. |
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