Evaluation of the enzymatic inhibition of Tyrosinase
| Regulatory reference: | Proprietary method |
| Claim: | Depigmenting action |
| Application field: | Active ingredients, mixtures or finished products. |
| Description of the test: | Evaluation of the efficacy of a sample in inhibiting the activity of the tyrosinase enzyme in a controlled in vitro direct reaction. |
Evaluation of the modulation of melanogenesis on pigmented cells
| Regulatory reference: | Proprietary method |
| Claim: | Skin whitening and Depigmenting action |
| Application field: | Active ingredients or finished products. |
| Description of the test: | Quantification of the inhibition of melanin synthesis in human melanoma cells exposed to the substance or product to be tested, in comparison with untreated controls. The same test can also be used to quantify an increase of melanin synthesis. |
Evaluation of the efficacy in the modulation of melanogenesis in pigmented cells by quantification of the expression of the tyrosinase gene
| Regulatory reference: | Proprietary method |
| Claim: | Skin whitening and Depigmenting action |
| Application field: | Active ingredients or finished products. |
| Description of the test: | Evaluation of the changes in the expression of the tyrosinase gene in human melanoma cells. |
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