Anti-wrinkle activity through skin profilometry
| Regulatory reference: | Proprietary method |
| Claim: | Anti-aging, Anti-wrinkle, Reduces signs of aging |
| Application field: | Raw materials and finished products. |
| Description of the test: | Blue laser skin profilometry: this technique allows you to carry out cosmetological studies of anti-wrinkle products with great reproducibility and accuracy thanks to a sophisticated instrumental and electronic set-up, which allows you to normalize the position of the volunteer and the test area in a millimetric way, an indispensable condition in conducting an anti-aging clinical study for the reduction of facial wrinkles. This clinical study allows a quantitative experimental evaluation of the anti-wrinkle effect through 2D measurements of wrinkles (Ra, Rz, LR, SPa, SPt, SPTm), and 3D (volume, average and maximum depth of wrinkles), and through quantitative morphological evaluations (mm2 of total surface covered by wrinkles). This allows the anti-wrinkle effect of any product to be assessed in the most appropriate way: immediate tensor effect, filler, firming effects (firming, toning, plumping, etc.) |
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Hyaluronic acid synthesis test
| Regulatory reference: | Proprietary method. |
| Claim: | Anti-aging and Reduces signs of aging |
| Application field: | Raw materials and finished products. |
| Description of the test: | In vitro evaluation of the synthesis of hyaluronic acid up to 48h following exposure to the sample, in several concentrations, on skin and dermal papillae fibroblasts. |
Evaluation of skin smothness, elasticity and compactness
| Regulatory reference: | Guidelines to Commission Regulation (EU) No 655/2013 |
| Claim: | Anti-aging, Reduces signs of aging and Illuminating |
| Application field: | Visage products for mature skin. |
| Description of the test: | Instrumental evaluation of R0, R2 and R9 parameters. Instrument: MPA580 Cutometer® for the determination of skin viscoelasticity. Manufacturer: Courage-Khazaka GmbH (Germany). The L* parameter is also quantified for skin brightness. The effect is quantified through reflection colorimetry using a CL-200 Chromameter Measurements of parameters L* and b* are performed before and after the application of the product. |
Quantification of the increase in proliferation and activation of protein synthesis on skin cells
| Regulatory reference: | Proprietary method |
| Claim: | Anti-aging and Redensifies and tones the epidermis |
| Application field: | Finished products and active ingredients. |
| Description of the test: | Quantitative evaluation of the efficacy of a product or active ingredient in increasing cell proliferation and protein synthesis. MTT test and protein quantification at different time intervals. The test can be performed on human keratinocytes or fibroblasts. |
