Anti-wrinkle activity through skin profilometry
| Regulatory reference: | Proprietary method |
| Claim: | Anti-aging, Anti-wrinkle, Reduces signs of aging |
| Application field: | Raw materials and finished products. |
| Description of the test: | Blue laser skin profilometry: this technique allows you to carry out cosmetological studies of anti-wrinkle products with great reproducibility and accuracy thanks to a sophisticated instrumental and electronic set-up, which allows you to normalize the position of the volunteer and the test area in a millimetric way, an indispensable condition in conducting an anti-aging clinical study for the reduction of facial wrinkles. This clinical study allows a quantitative experimental evaluation of the anti-wrinkle effect through 2D measurements of wrinkles (Ra, Rz, LR, SPa, SPt, SPTm), and 3D (volume, average and maximum depth of wrinkles), and through quantitative morphological evaluations (mm2 of total surface covered by wrinkles). This allows the anti-wrinkle effect of any product to be assessed in the most appropriate way: immediate tensor effect, filler, firming effects (firming, toning, plumping, etc.) |
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Posted in Anti-wrinkle, Anti age, Anti-aging, Reduces signs of aging, Claim for cosmetics
Hyaluronic acid synthesis test
| Regulatory reference: | Proprietary method. |
| Claim: | Anti-aging and Reduces signs of aging |
| Application field: | Raw materials and finished products. |
| Description of the test: | In vitro evaluation of the synthesis of hyaluronic acid up to 48h following exposure to the sample, in several concentrations, on skin and dermal papillae fibroblasts. |
Posted in Anti age, Anti-aging, Reduces signs of aging, Claim for cosmetics
In vitro test for the evaluation of the protective effect against air pollution
| Regulatory reference: | Proprietary method |
| Claim: | Anti-pollution and Antioxidant |
| Application field: | Raw materials and finished products. |
| Description of the test: | In vitro test for the evaluation of the protective effect against air pollution. The purpose of this test is to evaluate the efficacy of the sample in reducing ROS release in response to a mix of heavy metals and atmospheric dust particulate in a skin-derived cell model. |
Antioxidant efficacy test: ORAC Test
| Regulatory reference: | UE Regulations |
| Claim: | Antioxidant |
| Application field: | Active ingredients for cosmetics products and medical devices. |
| Description of the test: | Quantification of the antioxidant efficacy through ORAC (Oxygen Radical Absorbance Capacity) test. |
Posted in Anti age, Antioxidant, Claim for cosmetics
Evaluation of skin smothness, elasticity and compactness
| Regulatory reference: | Guidelines to Commission Regulation (EU) No 655/2013 |
| Claim: | Anti-aging, Reduces signs of aging and Illuminating |
| Application field: | Visage products for mature skin. |
| Description of the test: | Instrumental evaluation of R0, R2 and R9 parameters. Instrument: MPA580 Cutometer® for the determination of skin viscoelasticity. Manufacturer: Courage-Khazaka GmbH (Germany). The L* parameter is also quantified for skin brightness. The effect is quantified through reflection colorimetry using a CL-200 Chromameter Measurements of parameters L* and b* are performed before and after the application of the product. |
Posted in Anti age, Anti-aging, Illuminating, Reduces signs of aging, Claim for cosmetics
Evaluation of the activation of the synthesis of collagen and fibronectin
| Regulatory reference: | Proprietary method |
| Claim: | Activates the synthesis of collagen, elastin, fibronectin |
| Application field: | Finished products and active ingredients. |
| Description of the test: | Real time PCR analysis of the expression of collagen and fibronectin, compared to an untreated control, to determine the efficacy of a product or ingredient in activating the synthesis of these proteins involved in maintaining skin elasticity and prevent aging. |
Direct antioxidant efficacy assay
| Regulatory reference: | Proprietary method |
| Claim: | Antioxidant |
| Application field: | Finished products and substances. |
| Description of the test: | Colorimetric evaluation of the antioxidant activity of the sample in solution on a controlled oxidation reaction. Results in terms of percentage of decrease of oxidation processes caused by free oxygen radicals. |
Posted in Anti age, Antioxidant, Claim for cosmetics
Anti-radical and antioxidant efficacy test on skin cells
| Regulatory reference: | Proprietary method |
| Claim: | Antioxidant |
| Application field: | Raw ingredients or finished products. |
| Description of the test: | Quantification of the efficacy of the sample in inhibiting cytoplasmic oxidation phenomenon and protecting the skin cell model (immortalized human keratinocytes) from damaged caused by ROS. |
Posted in Anti age, Antioxidant, Claim for cosmetics
Evaluation of the activation of the synthesis of collagen and elastin
| Regulatory reference: | Proprietary method |
| Claim: | Activates the synthesis of collagen, elastin, fibronectin |
| Application field: | Finished products and active ingredients. |
| Description of the test: | Quantitative analysis of the effect of the tested product in increasing the synthesis of collagen and elastin. The test is performed on several concentration and time points using real time PCR on cultured cell lines. The test can be performed on human keratinocytes or fibroblasts. |
Quantification of the increase in proliferation and activation of protein synthesis on skin cells
| Regulatory reference: | Proprietary method |
| Claim: | Anti-aging and Redensifies and tones the epidermis |
| Application field: | Finished products and active ingredients. |
| Description of the test: | Quantitative evaluation of the efficacy of a product or active ingredient in increasing cell proliferation and protein synthesis. MTT test and protein quantification at different time intervals. The test can be performed on human keratinocytes or fibroblasts. |
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