H295R Steroidogenesis Assay – OECD 456
| Regulatory reference: | According to OECD 456. |
| Claim: | No endocrine disruption |
| Application field: | Raw ingredients and finished products. |
| Description of the test: | The purpose of the test is identifying substances that interfere with 17-ß estradiol and testosterone production. |
In vitro test for the evaluation of the protective effect against air pollution
| Regulatory reference: | Proprietary method |
| Claim: | Anti-pollution and Antioxidant |
| Application field: | Raw materials and finished products. |
| Description of the test: | In vitro test for the evaluation of the protective effect against air pollution. The purpose of this test is to evaluate the efficacy of the sample in reducing ROS release in response to a mix of heavy metals and atmospheric dust particulate in a skin-derived cell model. |
Cytotoxicity test for the identification of substances with acute oral toxicity LD50>2000 mg/kg
| Regulatory reference: | OECD 129: on using cytotoxicity tests to estimate starting doses for acute oral systemic toxicity tests; EURL-ECVAM 2011: follow-up study on the predictive capacity of the 3T3 Neutral Red Uptake cytotoxicity assay to correctly identify substances not classified for acute oral toxicity under the EU CLP system. |
| Claim: | Biocompatible and Non citotoxic |
| Application field: | Chemical substances or mixtures. |
| Description of the test: | The in vitro LD50 prediction test is designed to test chemicals or mixtures by evaluating their cytotoxic effect on an in vitro model of BALB/c 3T3 murine fibroblasts. |
Ocular irritation test on reconstructed corneal epithelium
| Regulatory reference: | OECD n° 492 |
| Claim: | Non-irritant for the eyes |
| Application field: | Chemical substances or mixtures. |
| Description of the test: | Identification of chemical substances or mixtures that do not require classification as (severe) irritants through evaluation of their cytotoxic effects on an in vitro reconstructed corneal epithelium model. NOTE: if the sample qualifies as irritant, further tests are required in order to determine the degree of irritant action. |
In vitro short term exposure test
| Regulatory reference: | OECD n° 491 |
| Claim: | Non-irritant for the eyes (CLP Labeling) |
| Application field: | Raw ingredients or finished products. |
| Description of the test: | Short term exposure analysis by application of each tested substance on SIRC (Statens Seruminstitut Rabbit Cornea) cells for the determination of the effect on cell viability through MTT test. |
Patch test for the evaluation of the irritation potential on 3D reconstructed epidermis
| Regulatory reference: | OECD n° 439 |
| Claim: | Non-irritant for the skin |
| Application field: | Chemical substances or mixtures. |
| Description of the test: | Patch test for the evaluation of the irritation potential of chemical substances or mixtures through evaluation of their cytotoxic effect on an in vitro reconstructed model of human skin. |
Antimicrobial activity test vs Propionibacterium acnes
| Regulatory reference: | Proprietary method |
| Claim: | Anti-acne |
| Application field: | Cosmetics and raw cosmetic ingredients. |
| Description of the test: | Evaluation of the anti-acne efficacy by testing the inhibition of the proliferation of Propionibacterium acnes by an ingredient or finished product. |
Posted in Acne, Anti-acne, Cosmetic raw materials
Quantification of trace substances and impurities
| Regulatory reference: | UE Regulation |
| Claim: | Titer of substances |
| Application field: | Cosmetics and medical devices. |
| Description of the test: | Analysis of trace substances, residues and impurities for quality control purposes through UPLC or GC-MS. Includes sample preparation. |
Quantification of the titer of substances
| Regulatory reference: | Regulation 1223/209 |
| Claim: | Titer of substances and Limit of preservatives |
| Application field: | Preservatives, dyes, sunscreen filters. |
| Description of the test: | Quantification method for the measurement of the titer of substances by HPLC, UPLC, GC-MS after preparative steps. |
OECD 442D in vitro sensitization test
| Regulatory reference: | OECD 442D |
| Claim: | No skin sensitizing and Hypoallergenic |
| Application field: | Substances or mixtures. |
| Description of the test: | The ARE-Nrf2 method employs cytofluorometry to quantify the antioxidant response in a culture of human keratinocytes after exposure to the product. |
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